ProSpec-OTOR Human/CYT-582

Otoraplin, Fibrocyte-derived protein, Melanoma inhibitory activity-like protein, OTOR, MIAL, FDP, MIAL1, MGC126737, MGC126739.

OTOR proteins is also known as fibrocyte-derived protein and Melanoma inhibitory activity-like . Otoraplin is a member of the melanoma-inhibiting activity gene family. Otoraplin is a secreted 16 kDa globular protein that is expressed in the inner ear by periotic mesenchyme and developing and mature fibrocytes. OTOR is highly homologous to MIA/cartilage-derived retinoic acid-sensitive protein , which is a cartilage-specific protein that is also expressed in malignant melanoma cells. The 111 amino acid mature human otoraplin contains 1 SH3 domain and a Tyr at position 50 that is reportedly sulfated. Otoraplin takes pasrt in the initiation of periotic mesenchyme chondrogenesis.
Otoraplin is secreted through the Golgi apparatus and plays a role in cartilage development and maintenance. A frequent polymorphism in the translation start codon of OTOR can abolish translation and may be associated with forms of deafness.

OTOR 蛋白也称为纤维细胞衍生蛋白和黑色素瘤抑制活性蛋白。 Otoraplin 是黑色素瘤抑制活性基因家族的成员。 Otoraplin 是一种分泌的 16 kDa 球状蛋白，在内耳中由周围间充质以及发育和成熟的纤维细胞表达。 OTOR 与 MIA/软骨衍生的视黄酸敏感蛋白高度同源，后者是一种软骨特异性蛋白，也在恶性黑色素瘤细胞中表达。 111 个氨基酸的成熟人 otoraplin 包含 1 个 SH3 结构域和据报道在 50 位被硫酸化的 Tyr。 Otoraplin 在启动 periotic 间充质软骨形成中发挥作用。
Otoraplin 通过高尔基体分泌，在软骨发育和维持中发挥作用。 OTOR 的翻译起始密码子中的频繁多态性可以消除翻译并可能与耳聋形式有关。

Otoraplin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12.7 kDa.
The OTOR is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Otoraplin Human Recombinant 是一种单一的、非糖基化的多肽链，包含 111 个氨基酸，分子量为 12.7 kDa。
OTOR 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The OTOR protein was lyophilized from a concentrated solution containing 20mM PBS pH-7.4 and 130mM NaCl.

It is recommended to reconstitute the lyophilized Otoraplin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Otoraplin，然后可以进一步稀释成其他水溶液。

Lyophilized OTOR Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OTOR should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 OTOR 重组体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，OTOR 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

VHGIFMDRLASKKLCADDECVYTISLASAQEDYNAPDCRFINVKKGQQIYVYS
KLVKENGAGEFWAGSVYGDGQDEMGVVGYFPRNLVKEQRVYQEATKEVPTT
DIDFFCE.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。