ProSpec-RELM g Mouse, His/CYT-455

Resistin-like gamma, RELMgamma,RELM-γ, RELM-g.

RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

RELM-γ是抵抗素样分子的新成员/发现于小鼠和大鼠的炎症区家族。 微阵列和实时 RT-PCR 实验揭示了香烟烟雾暴露与未处理大鼠的鼻呼吸道上皮细胞中 RELMgamma mRNA 的抑制。 生理组织特异性表达的分析揭示了造血组织中的最高表达，表明 RELM-γ 具有细胞因子样作用。 在骨髓、脾脏和肺以及外周血粒细胞中可检测到 RELM-γ-mRNA。 与模拟转染的细胞相比，用 RELM-γ 表达质粒转染的早幼粒细胞 HL60 细胞具有增加的增殖率，并显示出对视黄酸诱导的粒细胞分化的反应改变。 总之，这些数据提供了第一个实验证据，证明 RELM-γ 是一种具有受限表达模式的分泌分子，可能在早幼粒细胞分化中起作用。

RELM-gamma Mouse Recombinant is a His -Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues – HisTag .

RELM-gamma Mouse Recombinant 是一种带 His 标签的融合蛋白，分子量为 11 kDa，含有 RELM-gamma Mouse 的 86 个氨基酸残基和 16 个额外的氨基酸残基——HisTag。

Escherichia Coli.

Filtered White lyophilized powder.

Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入 0.1M 醋酸盐缓冲液 pH4 制备约 0.5 mg/ml 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10µg/ml 的浓度。 在较高浓度下，这种抗原的溶解度是有限的。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。
在 -20°C 下储存时，冻干蛋白在有效期内保持稳定。

Greater than 95% as determined by -PAGE.

MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.