ProSpec-Resistin Mouse, Flag/CYT-457

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们的敏感性。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且被禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化过程中增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Resistin Mouse is manufactured with signal sequence of phage fd and C-terminal fusion of flagTag . Resistin Mouse Recombinant Flag-Tagged Fusion Protein is 13.7 kDa protein containing 93 amino acid residues of the Resistin Mouse and 31 additional amino acid residues – signal sequence of phage fd, flagTag .

Resistin Mouse 是用 phage fd 的信号序列和 flagTag 的 C 端融合制造的。 Resistin Mouse Recombinant Flag-Tagged Fusion Protein 是一种 13.7 kDa 的蛋白质，含有 Resistin Mouse 的 93 个氨基酸残基和 31 个额外的氨基酸残基 – 噬菌体 fd，flagTag 的信号序列。

Escherichia Coli.

Filtered White lyophilized powder.

Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入 0.1M 醋酸盐缓冲液 pH4 配制约 0.5 mg/mL 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

MKKLLFAIPL VVPFYSHSTM ASMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVASLEDYKD DDDK.

Greater than 95% as determined by -PAGE.

Prospec’s products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。