ProSpec-Resistin Mutant Human/CYT-585

Resistin, Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

抵抗素、富含半胱氨酸的分泌蛋白 FIZZ3、脂肪组织特异性分泌因子、ADSF、C/EBP-ε-调节骨髓特异性分泌的富含半胱氨酸蛋白、富含半胱氨酸的分泌蛋白 A12-α 样 2、RSTN、XCP1、 RETN1、MGC126603、MGC126609。

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖和胰岛素抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们对胰岛素的敏感性。 Steppan 等人。已经提出抵抗素抑制胰岛素刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且通过禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并且受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化期间增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

9.9 kDa protein containing 93 amino acid residues, produced in E.coli. Mutant-Resistin has had a Cysteine residue mutated to prevent dimerization and possibly acts as an antagonist.

含有 93 个氨基酸残基的 9.9 kDa 蛋白质，在大肠杆菌中产生。 Mutant-Resistin 有一个半胱氨酸残基突变以防止二聚化并可能充当拮抗剂。

Escherichia Coli.

Recombinant Human Resistin was lyophilized from a concentrated solution containing 0.1% TFA.

Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

MSSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP.

Greater than 95% as determined by -PAGE.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。