ProSpec-SAA Human/CYT-942

Serum amyloid A protein which is a member of the SAA family is encoded by the SAA gene in humans. SAA is expressed by the liver and secreted in plasma. Serum amyloid A is the main acute phase reactant and apolipoprotein of the HDL complex. Elevated serum SAA protein levels may be linked with lung cancer. The level of Apo-SAA, which is ordinarily 1-5 ?g/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL Apolipoprotein.

作为SAA家族成员的血清淀粉样蛋白A由人类的SAA基因编码。 SAA由肝脏表达并分泌到血浆中。 血清淀粉样蛋白 A 是 HDL 复合物的主要急性期反应物和载脂蛋白。 血清SAA蛋白水平升高可能与肺癌有关。 血浆中的 Apo-SAA 水平通常为 1-5 μg/ml，在炎症刺激后 24 小时内增加 500-1000 倍，在这些条件下，它是最丰富的 HDL 载脂蛋白。

APO-SAA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa. Recombinant Apo-SAA is a consensus SAA molecule corresponding to human Apo-SAA1a, except for the presence of an N-terminal methionine, the substitution of asparagine for aspartic acid at position 60, and arginine for histidine at position 71 .
The Human APO-SAA is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 APO-SAA Human Recombinant 是一条单一的、非糖基化的多肽链，包含 104 个氨基酸，分子量为 11.7kDa。 重组 Apo-SAA 是一种与人 Apo-SAA1a 相对应的共有 SAA 分子，除了存在一个 N 端甲硫氨酸、60 位天冬氨酸取代天冬氨酸和 71 位精氨酸取代组氨酸。
人 APO-SAA 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Human SAA was lyophilized from a concentrated solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

It is recommended to reconstitute the lyophilized SAA Human in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干的 SAA Human，然后可以进一步稀释成其他水溶液。

Lyophilized Human SAA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human SAA should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的人 SAA 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，人 SAA 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISN ARENIQRFFG RGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.

The biological activity determined by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4µM.

由其下调主动脉平滑肌细胞中脂质生物合成的能力决定的生物活性。 发现有效浓度为4μM。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.