ProSpec-Thrombopoietin Human/CYT-1178,Thrombopoietin Human Recombinant

Thrombopoietin Human

Thrombopoietin Human Recombinant
CYT-1178
2µg
10µg
1mg

Catalogue number

CYT-1178

Synonyms

Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.

血小板生成素是一种糖蛋白激素，主要由肝脏和肾脏产生，可调节骨髓的血小板生成。 TPO 刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Description

TPO Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain containing 343 amino acids and having a molecular mass of 36.8kDa.
TPO is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 HEK293 细胞中产生的 TPO Human Recombinant 是单条糖基化多肽链，含有 343 个氨基酸，分子量为 36.8kDa。
TPO 在 C 端与 6 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

HEK293 cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TPO protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

The ED50 range is ≤10ng/ml. It is measured by cell proliferation assay using MO7e human megakaryocytic leukemic cells.

Amino acid sequence

DGSHMSPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEGHHH HHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.