ProSpec-TFF2 Human/CYT-612,Trefoil Factor-2 Human Recombinant

TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains.

TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠黏膜中表达的稳定分泌蛋白，可保护黏膜免受损伤、稳定黏液层并影响上皮细胞的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。

TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids and having a total molecular mass of 12 kDa. TFF2 Human Recombinant includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds and was purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TFF-2 Human Recombinant 是单一的、非糖基化的多肽链，包含 106 个氨基酸，总分子量为 12 kDa。 TFF2 Human Recombinant 包含一个 40 个氨基酸的三叶基序，其中包含三个保守的分子内二硫键，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The TFF2 protein was lyophilized from 0.4μm filtered solution at a concentration of 1mg/mL containing 1x PBS pH-7.4.

It is recommended to reconstitute the lyophilized TFF2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TFF2，然后可以进一步稀释成其他水溶液。

Lyophilized TFF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF2 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TFF2 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TFF2 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.

EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。