TFF2 Human, His
Trefoil Factor-2 Human Recombinant, His Tag
CYT-611
5µg
20µg
1mg
Trefoil Factor-2 Human Recombinant, His Tag
CYT-611
5µg
20µg
1mg
Catalogue number
CYT-611
Synonyms
TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.
Introduction
Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝,这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠黏膜中表达的稳定分泌蛋白,可保护黏膜免受损伤、稳定黏液层并影响上皮细胞的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。
Description
TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 13.2 kDa.
TFF2 Human Recombinant is purified by proprietary chromatographic techniques.
TFF2 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-2 Human Recombinant 是一个单一的、非糖基化的多肽链,含有 116 个氨基酸,其中包括一个在 N 端融合的 10 个氨基酸的 His 标签,总分子量为 13.2 kDa。
TFF2 Human Recombinant 通过专有的色谱技术进行纯化。
TFF2 Human Recombinant 通过专有的色谱技术进行纯化。
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized powder.
Formulation
The TFF2 protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5, and 20mM NaCl.
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液,让冻干颗粒完全溶解。 产品不是无菌的! 在细胞培养中使用之前,请用适当的无菌过滤器过滤产品。
Stability
Lyophilized TFF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF2 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF2 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,TFF2 应在 4°C 下储存 2-7 天,以备将来使用
-18°C。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。
-18°C。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。
Purity
Greater than 95.0% as determined by -PAGE.
Amino acid sequence
MKHHHHHHAS EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
-
TFF1 Human
-
TFF3 Human
-
TFF2 Human
-
TFF1 Human, His
-
TFF3 Human, His