ProSpec-TFF3 Human/CYT-005,Trefoil Factor-3 Human Recombinant

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.

TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TFF-3 Human Recombinant 是一种同型二聚体、非糖基化多肽链，含有 2 x 59 个氨基酸链，其中包括一个 40 个氨基酸的三叶基序，该基序含有 3 个保守的分子间二硫键，总分子量为 13.2kDa .
TFF-3 Human Recombinant 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TFF3，然后可以进一步稀释成其他水溶液。

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TFF3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TFF3 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by RP-HPLC and -PAGE analysis.

EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.

The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10µg/ml, corresponding to a specific activity of > 100IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.