ProSpec-TFF3 Rat/CYT-781,Trefoil Factor-3 Rat Recombinant

Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf.

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.

TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of Flag-Tag . The TFF3 Rat produced in E.coli, is 7.7kDa protein containing a total of 68 amino acid residues.

Trefoil Factor-3 Rat 作为重组蛋白构建，具有 9 a.a C 末端融合的 Flag-Tag。 在大肠杆菌中产生的 TFF3 大鼠是 7.7kDa 的蛋白质，总共含有 68 个氨基酸残基。

Escherichia Coli.

Filtered White lyophilized powder.

TFF3 protein filtered and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 7.5.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Greater than 95.0% as determined by -PAGE.

MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.