ProSpec-TSLP Human/CYT-572,Thymic Stromal Lymphopoietin Human Recombinant

Thymic Stromal Lymphopoietin, TSLP.

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.

TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。 TSLP 的生物活性与 IL-7 相关，并与由 IL-7 受体 α 链和 TSLPR 组成的异二聚体受体复合物结合。与 IL-7 类似，TSLP 增强 STAT3 和 STAT5 的磷酸化，但使用不包括 JAK 的激酶进行激活。 TSLP 诱导 T 细胞吸引趋化因子如 TARC 和 MDC 从单核细胞中释放并触发 CD11c 树突状细胞。 TSLP 激活的树突状细胞启动幼稚 T 细胞以制造促过敏细胞因子，如 Iinterleukin-4、Interleukin-5、Interleukin-13 和 TNF-α，而下调 Interleukin-10 和 IFN-γ 在过敏的起始中发挥作用炎。

TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.
The TSLP is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TSLP Human Recombinant 是单一的非糖基化多肽链，包含 132 个氨基酸，分子量为 15 kDa。
TSLP 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution contains 130mM NaCl, and 20mM sodium phosphate, pH 7.4.

It is recommended to reconstitute the lyophilized TSLP in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TSLP，然后可以进一步稀释成其他水溶液。

Lyophilized TSLP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TSLP should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TSLP 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后 TSLP 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ.

The ED50 is determined by the ability to induce proliferation of human Interleukin-7 receptor alpha & human TSLP Receptor co-transfected with mouse BaF3 cells which was found to be less than 0.3ng/ml.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.