ProSpec-TSLP Human, His/CYT-836,Thymic Stromal Lymphopoietin Human Recombinant, His Tag

Thymic Stromal Lymphopoietin, TSLP.

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.

TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。 TSLP 的生物活性与 IL-7 相关，并与由 IL-7 受体 α 链和 TSLPR 组成的异二聚体受体复合物结合。与 IL-7 类似，TSLP 增强 STAT3 和 STAT5 的磷酸化，但使用不包括 JAK 的激酶进行激活。 TSLP 诱导 T 细胞吸引趋化因子如 TARC 和 MDC 从单核细胞中释放并触发 CD11c 树突状细胞。 TSLP 激活的树突状细胞启动幼稚 T 细胞以制造促过敏细胞因子，如 Iinterleukin-4、Interleukin-5、Interleukin-13 和 TNF-α，而下调 Interleukin-10 和 IFN-γ 在过敏的起始中发挥作用炎。

TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 16.2kDa.

在大肠杆菌中产生的 TSLP Human Recombinant 是一种单一的、非糖基化的多肽链，含有 141 个氨基酸，包括 N 端的 10 个氨基酸的 His 标签。 总计算分子量为 16.2kDa。

Escherichia Coli.

Filtered White lyophilized powder.

TSLP was filtered and lyophilized in phosphate buffered saline.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. TSLP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 TSLP 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间。

MKHHHHHHASYDFTNCDFEK IKAAYLSTIS KDLITYMSGT KSTEFNNTVS CSNRPHCLTE IQSLTFNPTA GCASLAKEMF AMKTKAALAI WCPGYSETQI NATQAMKKRR KRKVTTNKCL EQVSQLQGLW RRFNRPLLKQ Q.

Greater than 95.0% as determined by -PAGE.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.