ProSpec-TNF a Human, Sf9/CYT-903,Tumor Necrosis Factor-alpha Human Recombinant, Sf9

TNF a Human, Sf9
Tumor Necrosis Factor-alpha Human Recombinant, Sf9
CYT-903
2µg
10µg
1mg

Catalogue number

CYT-903

Synonyms

Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, Tumor Necrosis Factor , Tumor Necrosis Factor-Alpha, TNF, Macrophage-Derived, TNF, Monocyte-Derived, TNF Superfamily, APC1 Protein, Member 2, DIF, Tumor necrosis factor.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF a produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 163 amino acids and having a molecular mass of 18.1kDa.

TNF a is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNF a 是单条糖基化多肽链，含有 163 个氨基酸，分子量为 18.1kDa。

TNF a 在 C 末端用 6 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNF a protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 90.0% as determined by-PAGE.

Biological Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

The ED50 for this effect is ≤ 0.2 ng/ml.

在代谢抑制剂放线菌素 D 存在下，使用 L929 小鼠纤维肉瘤细胞在细胞毒性试验中测量。

这种效应的 ED50 ≤ 0.2 ng/ml。

Amino acid sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.