ProSpec-TNFA Rat, His Active/CYT-1057,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag Active

Tumor Necrosis Factor-alpha, TNF a His,  Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

肿瘤坏死因子-α、TNF a His、Cachectin、TNF-α、肿瘤坏死因子配体超家族成员 2、TNF-a、N 端片段、NTF、细胞内结构域 1、细胞内结构域 2、ICD2、C 结构域 1、 C 域 2，肿瘤坏死因子，可溶性形式，Tnfa，Tnfsf2，RATTNF，Tnfa。

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

TNFA Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 19.9kDa.
TNFA Rat is expressed with an 25 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFA Rat Recombinant 是一条单一的、非糖基化的多肽链，含有 181 个氨基酸，分子量为 19.9kDa。
TNFA Rat 在 C 末端用 25 个氨基酸的 His 标签表达，并通过专有色谱技术进行纯化。

TNFA Rat protein solution contains Phosphate Buffered Saline , 10% glycerol and 1mM DTT.

Greater than 90.0% as determined by -PAGE.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is ≤ to 0.2 ng/ml.

MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.