ProSpec-TNFR2 Human/CYT-769,Tumor Necrosis Factor Receptor Type 2 Human Recombinant

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNFR2.

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.

TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体，对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外，在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物，介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集，它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知；然而，据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

TNFR2 Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20kDa. The TNFR2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR2 Human 是一条单一的、非糖基化的多肽链，含有 184 个氨基酸，分子量为 20kDa。 TNFR2 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFR2 was Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR2，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR2 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后 TNFR2 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by -PAGE.

MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.

The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2µg/ml, corresponding to a specific activity of > 5000IU/mg in the presence of 0.25ng/mL of rHuTNF-a.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.