ProSpec-TNFRSF14 Human/CYT-821 | Lumiprobe官网中国授权代理商

TNFRSF14 Human

HVEM-Fc Human Recombinant
CYT-821
ProSpec-TNFRSF14 Human/CYT-821 20µg
ProSpec-TNFRSF14 Human/CYT-821 100µg
ProSpec-TNFRSF14 Human/CYT-821 1mg

Catalogue number

CYT-821

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 14, HVEM, TR2, Herpes Virus Entry Mediator A, Tumor Necrosis Factor Receptor-Like 2, Herpesvirus Entry Mediator, HVEA, ATAR, CD270, LIGHTR, CD40-Like Protein, Tumor Necrosis Factor Receptor-Like Gene2.

Introduction

TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. TNFRSF14 is expressed in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. TNFRSF14 operates as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Additionally, TNFRSF14 encourages the proliferation of T cells, and triggers apoptosis of various tumor cells.

TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。此外，TNFRSF14 促进 T 细胞的增殖，并引发各种肿瘤细胞的凋亡。

Description

TNFRSF14 Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 396 amino acids and having a molecular mass of 58.0kDa.

However, TNFRSF14 migrates with an apparent molecular mass of 70 kDa in -PAGE under reducing conditions.

Pichia Pastoris 生产的 TNFRSF14 人重组体是单条糖基化多肽链，包含 396 个氨基酸，分子量为 58.0kDa。
然而，在还原条件下，TNFRSF14 在 -PAGE 中以 70 kDa 的表观分子量迁移。

Source

Pichia Pastoris.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFRSF14 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4, with 3 % Trehalose.

Solubility

It is recommended to reconstitute the lyophilized TNFRSF14 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TNFRSF14，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFRSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 TNFRSF14 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TNFRSF14 应在 4°C 下储存 2-7 天，以备将来在低于 -18°C 的温度下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:

Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGTLEE CQHQTKCSWL VTKAGAGTSS SHWVEPKSSD KTHTCPPCPA PEFEGAPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPTP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

Biological Activity

Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit TNF-beta -mediated cytotoxicity using murine L929 cells.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.