Hampton蛋白结晶试剂盒HEPES Buffer

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Products > Optimize Reagents > Optimize – Buffers > HEPES Buffer

HEPES Buffer

应用

  • Crystallization grade HEPES buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 HEPES 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 HEPES

Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14,4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity ≥ 99.5%
pKa 7.5 at 25°C

Useful pH range of HEPES buffer 6.8 – 8.2

别名：2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic 酸）
C8H18N2O4S
238.31 先生
CAS 编号 [7365-45-9]
欧盟编号 230-907-9
贝尔斯坦注册号 883043
默克 14,4654
RTECS TL6809000
MDL 编号 MFCD00006158
PubChem 物质 ID 24895616
纯度≥99.5%
pKa 7.5 在 25°C

HEPES 缓冲液的有用 pH 范围 6.8 – 8.2

Titrate HR2-585 to the desired pH between 6.8 – 8.2 using NaOH
HR2-585 Measured pH Range: 5.4 – 5.5 at 25°C
HR2-585 Measured Conductivity Range: 160.1 – 236.9 μS/cm at 25°C
HR2-585 Measured Refractive Index Range: 1.37093 – 1.37131 at 20°C

HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 – 7.0 mS/cm at 25°C
HR2-785 Measured Refractive Index Range: 1.37133 – 1.37176 at 20°C

HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 – 13.6 mS/cm at 25°C
HR2-729 Measured Refractive Index Range: 1.37177 – 1.37290 at 20°C

Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO4 0.005% max

HEPES Buffer
CAT NO

HR2-585

NAME

1.0 M HEPES

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-785

NAME

1.0 M HEPES pH 7.0

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-729

NAME

1.0 M HEPES pH 7.5

DESCRIPTION

100 mL

PRICE

$113.00

相关项目

  • Sodium hydroxide
  • StockOptions Hepes Buffer Kit
  • Individual StockOptions HEPES Reagents
  • 氢氧化钠
    StockOptions Hepes 缓冲液套件
    个别股票期权 HEPES 试剂

参考

1. Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995.

1. 来自北大西洋鲑鱼 (Salmo salar) 的天然弹性蛋白酶的结晶和初步 X 射线晶体学研究。 伯格伦德，GI； 斯纳拉斯，AO； 汉森，LK。 晶体学学报 D 51, 393-394, 1995。